Department of Anatomical sciences and cell Biology

MSc course outline Code 08

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Course Title: Techniques of Macro- and Micro-anatomy

Prerequisite: None

Credits: 2 (0.5 theory, 1.5 practical)

Course type: Theory-practical

Objective: Recognition of the function of anatomical sciences laboratories and preparing anatomical samples

 

Course outline:

9 hours theory:

·         Principles of medical and professional ethics in relation to corpses and tissue samples (one hour)

·         Work safety in laboratories (one hour)

·         Disposal of harmful chemicals (toxins, radioactivity and flammable) (one hour)

·         Preparing fixative solutions for human bodies, animal tissues and perfusion, preparing cross-sections and principle of tissue staining (2 hours)

·         Methods of preserving laboratory animals (2 hours)

·         Methods of preserving tissue samples and large human sample (museums) (2 hours)

51 hours practical:

·         Preparing solutions and determining pH and its concentration (2 hours)

·         Performing perfusion on laboratory animals (2 hours)

·         Corpse fixation (4 hours)

·         Preparing tissue slides, including tissue and fetal cross-sections, and sperm and blood smears (20 hours)

·         Staining tissue slides (Acridine Orange, Trichrome Mallory, Trichrome Masson, PAS, H&E) (9 hours)

·         Working with an optical microscope, florescent, and reading electron images (5 hours)

·         Familiarization with instruments of corpse dissection (2 hours)

·         Recognizing the methods of cell and tissue culture and bio-staining (5 hours)

·         Recognition of morphometric principles (2 hours)

Main References: latest edition approved by the professor and department

Student Evaluation Method:

Student-based principles will be observed during the term to evaluate the performance of students by the assigned projects, as well as oral and written final exams.